This chapter covers a range of viruses and viral diseases in which immunoassays play a role. The viruses included are: cytomegalovirus, Epstein-Barr virus, herpes simplex virus (HSV), HSV-1, HSV-2, dengue virus, rubella virus, measles, mumps, varicella-zoster, human T-cell leukemia virus, parvovirus B19, West Nile virus, rotavirus, and adenovirus. For each virus the etiologic agent and pathogenesis are described, followed by the principles involved in diagnosis and the typical assay technology involved. The following diseases are included in the virus sections: infectious mononucleosis, Burkitt lymphoma, post-transplant lymphoproliferative disorder, herpes, dengue hemorrhagic fever, dengue shock syndrome, rubella (German measles), congenital rubella syndrome, measles, mumps, varicella, T-cell leukemia and lymphoma, erythema infectosum, Fifth’s disease and fetal hydrops.

Carey-Ann Burnham is an Assistant Professor of Pathology & Immunology at Washington University School of Medicine in St. Louis and the Medical Director of Clinical Microbiology for Barnes Jewish Hospital, St. Louis, MO. She is the program director for the CPEP Fellowship in Clinical Microbiology at Washington University. Her research interests are improved methods for diagnostic microbiology, the transmission and epidemiology of community-associated Staphylococcus aureus, and Clostridium difficile. She is on the editorial board for the Journal of Clinical Microbiology and is the 2013 recipient of the American Society for Microbiology Siemens Healthcare Diagnostics Young Investigator Award.

Christopher Doern, PhD D(ABMM) is a board certified medical microbiologist who directs the clinical microbiology laboratory at Children's Medical Center of Dallas. He is an assistant professor of pathology at the University of Texas Southwestern Medical Center Dallas. Prior to taking his position as medical director of clinical microbiology, Dr. Doern completed a fellowship in medical and public health microbiology at the Washington University School of Medicine in St. Louis.

Steven Binder is the Senior Director of Technical Development for the Clinical Diagnostic Group at Bio-Rad Laboratories. He joined Bio-Rad in 1983, where he initially developed and introduced clinical chromatography methods used for catecholamine measurement and hemoglobinopathy screening. He served Bio-Rad as R&D manager for clinical chromatography from 1988 to 1998 and was closely involved in the development of new methods for diabetes monitoring and clinical toxicology. From 1998-2005, he led the development of a fully automated platform for multiplex immunoassay, with an emphasis on autoimmune and infectious diseases. His current work involves the evaluation of novel multiplex and digital technologies, as well as validation and commercial development of new biomarkers.
Steve received a B.A. in History and Science, magna cum laude, from Harvard University. He has authored over 25 papers in peer-reviewed journals and has received 11 US patents.

Herpesvirus, hemagglutination inhibition, enzyme-linked immunosorbent assay, IgM antibody capture, lateral flow, infectious mononucleosis, Burkitt lymphoma, post-transplant lymphoproliferative disorder, herpes, dengue hemorrhagic fever, dengue shock syndrome, rubella, German measles, congenital rubella syndrome, measles, mumps, varicella, T-cell leukemia, T-cell lymphoma, erythema infectosum, Fifth’s disease, fetal hydrops, cytomegalovirus, Epstein-Barr virus, herpes simplex virus (HSV), HSV-1, HSV-2, dengue virus, rubella virus, varicella-zoster virus, human T-cell leukemia virus, parvovirus B19, West Nile virus, rotavirus, adenovirus.